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Online Grootte-uitsluiting en ionenuitwisselingschromatografie op een SAX Beamline
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
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Online Grootte-uitsluiting en ionenuitwisselingschromatografie op een SAX Beamline

16,895 Views

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11:09 min

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January 05, 2017

DOI:

10.3791/54861-v

DOI:
10.3791/54861-v

•

11:09 min
January 05, 2017

•

5 Views
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1Structural Biology Group,European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences,Keele University, 4Groupe de Microscopie Electronique et Méthodes,Institut de Biologie Structurale

Summary

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De bepaling van de oplossing structuur van een eiwit door kleine hoek röntgen verstrooiing (SAXS) vereist monodisperse monsters. Hier presenteren we twee mogelijkheden te verzekeren minimale vertragingen tussen monstervoorbereiding en data acquisitie: online size-exclusion chromatography (SEC) en online ionenuitwisselingschromatografie (IEC).

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Cite this Article

Brennich, M. E., Round, A. R., Hutin, S. Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline. J. Vis. Exp. (119), e54861, doi:10.3791/54861 (2017).

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