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可视化痰生物膜发展影响使用腔室盖玻片型号
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model
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可视化痰生物膜发展影响使用腔室盖玻片型号

8,208 Views

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05:03 min

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December 14, 2016

DOI:

10.3791/54819-v

DOI:
10.3791/54819-v

•

05:03 min
December 14, 2016

•

6 Views
, , ,
1Physiology and Experimental Medicine,Hospital for Sick Children, 2Department of Clinical Microbiology,Royal College of Surgeons in Ireland, 3Department of Laboratory Medicine and Pathobiology,University of Toronto, 4Department of Pediatrics,University of Toronto

Summary

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This protocol describes the visualization of biofilm development following exposure to host-factors using a slide chamber model. This model allows for direct visualization of biofilm development as well as analysis of biofilm parameters using computer software programs.

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BiofilmSputumAntibioticsBacterial BiofilmsBiofilm DevelopmentChambered CoverglassMicroscopyViability StainingLuria BrothOptical DensityCentrifugationFiltrationPBS

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Beaudoin, T., Kennedy, S., Yau, Y., Waters, V. Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model. J. Vis. Exp. (118), e54819, doi:10.3791/54819 (2016).

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