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Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning
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Neuroscienze
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JoVE Journal Neuroscienze
Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning

Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning

DOI:
10.3791/54780-v

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15:53 min

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December 06, 2016

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, , , , , , , , , ,
1Translational Neuroradiology Section,National Institute of Neurological Disorders and Stroke, 2Cerebral Microcirculation Section,National Institute of Neurological Disorders and Stroke, 3Viral Immunology Section,National Institute of Neurological Disorders and Stroke

Capitoli

  • 00:00Titolo
  • 00:36MRI Preparation and Acquisition
  • 01:53Extracting Brain Surface
  • 04:06Selecting Slice Location
  • 05:05Creating MRI Blade Map
  • 05:46Imaging Brain and Blade Gap Surfaces
  • 07:27Editing Brain Contours
  • 07:50Creating the Brain Slicer Box
  • 11:25Printing the Brain Slicer Box
  • 12:16Cutting the Brain
  • 13:16Representative Results
  • 15:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

The overall goal of this protocol is to accurately align magnetic resonance imaging (MRI) image volumes with histology sections via the creation of customized 3D-printed brain holders and slicer boxes.

Tags

3D PrintingMRIHistologyBrain SectioningMarmosetFormalinFomblinT2-weighted ImagingImage ProcessingNoise ReductionSegmentationBinary MaskMorphological OperationsSurface Extraction

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