È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Ecology and Environmental Sciences,Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment,Kobe University, 3Faculty of Science and Technology,Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies,University of Hyogo
Capitoli
- 00:05Titolo
- 00:46Assembly of the Filtration System
- 01:37Filtration of Water Samples ≤4 L Using the Filter Cartridge
- 03:05Filtration of Water Samples >4 L Using the Filter Cartridge
- 04:23Extraction of eDNA from the Filter
- 06:57Results: MiFish Metabarcoding of eDNA
- 07:23Conclusion
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
