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Genetics

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JoVE Journal Genetics
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
 

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Article DOI: 10.3791/54673-v • 06:53 min • November 11th, 2016
November 11th, 2016 •
*1, *2, *1, 1
1Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center, University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3), 2Population Epidemiology Unit (UEP), Community Medicine, Geneva University Hospital
* These authors contributed equally

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Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.

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Circadian Clock Gene Expression Hormone Secretion Human Primary Cell Cultures Parallel Measurement Endocrine Cells Circadian Bioluminescence Secretory Activity Cell-autonomous Circadian Clocks Luciferin Reporters Lentiviral Transduction Growth Medium Synchronization Adenylyl Cyclase Activator Light-sensitive Organisms
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