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Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
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Biologia
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JoVE Journal Biologia
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

DOI:
10.3791/54555-v

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09:32 min

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October 23, 2016

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, ,
1Department of Physiology and Biophysics,University of Colorado Anschutz Medical Campus, 2Department of Bioengineering,University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology,University of Colorado Anschutz Medical Campus

Capitoli

  • 00:05Titolo
  • 00:44Sinoatrial Node Isolation
  • 04:25Sinoatrial Node Digestion and Sinoatrial Node Myocyte Dissociation
  • 06:29Sinoatrial Node Calcium Re-adaptation
  • 07:36Results: Electrophysiological Characterization of Cultured Sinoatrial Myocytes
  • 08:27Conclusion

Summary

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Methods are demonstrated for the isolation of sinoatrial node myocytes (SAMs) from adult mice for patch clamp electrophysiology or imaging studies. Isolated cells can be used directly or can be maintained in culture to permit expression of proteins of interest, such as genetically encoded reporters.

Tags

Keywords: Sinoatrial Node MyocytesCardiac PacemakingElectrophysiologyCalcium ReleaseMouseHeart DissectionAtriumTyrode’s SolutionHeparin

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