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Ein Ansatz zur Verbesserung der Ausrichtung und Myelinisierung von Dorsalwurzelganglion Neurons
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Ein Ansatz zur Verbesserung der Ausrichtung und Myelinisierung von Dorsalwurzelganglion Neurons
Capitoli
- 00:05Titolo
- 00:41Preparation of Pre-stretched Anisotropic Surface
- 02:22Isolation of DRG
- 05:07Culture of DRG on Pre-stretched Surface
- 06:12Co-culture of Schwann Cells with DRG Neurons on Pre-stretched Surface
- 07:54Results: Co-cultured Schwann Cells with Aligned DRG Axons on Stretched vs. Unstretched PDMS Substrates
- 08:42Conclusion
This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.
