Rivista
/
Ambiente
/
リアルタイムPCRによる沿岸堆積物および定量16SのrRNA遺伝子と転写物からの同時DNA-RNA抽出
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Please note that all translations are automatically generated. Click here for the English version.

リアルタイムPCRによる沿岸堆積物および定量16SのrRNA遺伝子と転写物からの同時DNA-RNA抽出

17,102 Views

•

11:37 min

•

June 11, 2016

DOI:

10.3791/54067-v

DOI:
10.3791/54067-v

•

11:37 min
June 11, 2016

•

13 Views
, , ,
1Microbiology, School of Natural Sciences,National University of Ireland Galway, 2School of Biological Sciences,University of Essex

Summary

Automatically generated

A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

Explore More Videos

DNA-RNA ExtractionCoastal Sediments16S RRNA Genes16S RRNA TranscriptsQuantitative PCRMolecular AnalysisMicrobial AbundanceMicrobial Diversity

Read Article

Cite this Article

Tatti, E., McKew, B. A., Whitby, C., Smith, C. J. Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR. J. Vis. Exp. (112), e54067, doi:10.3791/54067 (2016).

Download .ris file

Copy

Share Video

.

Copy