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Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats
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JoVE Journal Neuroscienze
Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

DOI:
10.3791/53897-v

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08:07 min

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August 24, 2016

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, ,
1Behavioral and Cognitive Neuroscience Cluster, Psychology Doctoral Program, The Graduate Center,CUNY, New York, NY, 2Department of Psychology, Queens College,CUNY, Flushing, NY, 3Behavioral and Cognitive Neuroscience Cluster, Psychology Doctoral Program, The Graduate Center,CUNY, Flushing, NY

Capitoli

  • 00:05Titolo
  • 00:54Testing Apparatus and Intake Procedures
  • 02:06Tissue Preparation
  • 03:22c-fos Staining Procedure
  • 05:29Determination of c-fos Immunoreactive Counts
  • 06:45Results: Actual Amygdala and Ventral Tegmental Area c-fox Activation Following Fats and Sugars
  • 07:35Conclusion

Summary

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The goal of this study is to identify reward-related distributed brain networks by delineating a reliable immunohistological technique using cellular c-fos activation to measure simultaneous changes in dopamine pathways and terminal sites after novel ingestion of fat and sugar in rats.

Tags

C-Fos ActivationMesolimbicMesocorticalDopamineRewardSugarFatIngestionVTABrainMotivationPalatable StimuliBehavioral AssaysCellular ActivationStatistical AnalysisFood RestrictionSaccharineIntake TestBrain DissectionParaformaldehydeSucrose SolutionOlfactory Bulb

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