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Immunokleuring Phospho-epitopen in gecilieerde Organen van Whole Mount zebravis embryo's
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Chimica
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JoVE Journal Chimica
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
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Immunokleuring Phospho-epitopen in gecilieerde Organen van Whole Mount zebravis embryo's

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08:42 min

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February 19, 2016

DOI:

10.3791/53747-v

DOI:
10.3791/53747-v

•

08:42 min
February 19, 2016

•

1 Views
, ,
1Department of Biology,Virginia Commonwealth University, 2Center for Human Disease Modeling,Duke University Medical Center

Summary

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Technieken worden beschreven aan fosfo-epitopen geheel zebravis embryo immunostain en voert vervolgens twee kleuren fluorescerende confocale lokalisatie in cellulaire structuren zo klein als primaire cilia. De technieken voor de vaststelling en beeldvorming kan de locatie en de kinetiek van het uiterlijk of interactie met specifieke eiwitten te bepalen.

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Cite this Article

Rothschild, S. C., Francescatto, L., Tombes, R. M. Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos. J. Vis. Exp. (108), e53747, doi:10.3791/53747 (2016).

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