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Neuroscience

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JoVE Journal Neuroscience
Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
 

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

Article DOI: 10.3791/53673-v • 09:54 min • February 26th, 2016
February 26th, 2016 •
1,2, 1, 1,2
1Brain Structure and Function Group, Neuroscience Research Australia, 2School of Medical Sciences, The University of New South Wales

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Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

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Keywords: Serotonergic Fibers Mouse Spinal Cord CLARITY/CUBIC Technique 3D Neuroanatomy Histology Central Nervous System Cardiovascular System Tissue Clearing Hydrogel Perfusion Tissue Harvesting Spinal Cord Dissection
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