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成像的CD4 T细胞间质迁移发炎真皮
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
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成像的CD4 T细胞间质迁移发炎真皮

DOI:
10.3791/53585-v

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11:28 min

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March 25, 2016

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, ,
1David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, Rochester, NY

Capitoli

  • 00:05Titolo
  • 00:56Effector T Cell Injection
  • 02:28Mouse Imaging Preparation
  • 05:21In vivo Time-lapse Imaging and Intravenous Antibody Administration
  • 08:51Results: Representative In Situ Adoptively Trabsferred Effector T Cell Imaging
  • 10:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

支配CD4效应T细胞的间质动力在炎症部位的机制比较陌生。我们提出了一种非侵入性的方式来可视化和体外 -primed CD4T细胞操纵在发炎耳朵真皮，允许这些细胞在原位的动态行为的研究。

Tags

CD4 T CellsInterstitial MigrationInflamed DermisIn Situ ImagingEffector T CellsFluorescent LabelingTail Vein InjectionFluorescent EmulsionEar DermisAnesthetizationTail Vein CatheterizationImmunologyImmune Cell Dynamics

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