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Deacetylierung Assays zu entwirren, das Zusammenspiel zwischen Sirtuine (SIRT2) und spezifische Protein-Substrate
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14:32 min
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February 27, 2016
DOI:
1Laboratory for Molecular Cancer Biology, Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center,Feinberg School of Medicine, Northwestern University, 2Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center,Feinberg School of Medicine, Northwestern University
Summary
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This protocol describes the required steps to execute in vitro and in vivo deacetylation assays in order to establish the role of proteins as specific deacetylation substrates for sirtuins and further study the role of reversible - lysine acetylation as a post-translational modification.
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Song, H. Y., Park, S., Kang, H., Vassilopoulos, A. Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates. J. Vis. Exp. (108), e53563, doi:10.3791/53563 (2016).
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