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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

DOI:
10.3791/53411-v

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09:38 min

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January 21, 2016

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, , , ,
1Department of Biological Sciences,Macquarie University, 2Vital-IT group,Swiss Institute of Bioinformatics, 3Laboratory of Microbiology,University of Neuchatel

Capitoli

  • 00:05Titolo
  • 01:01Separation of Biomass from Sediment
  • 02:36Collection of Biomass on Filter Membrane
  • 03:31Lysis of Vegetative Cells
  • 05:44Dnase Treatment
  • 07:02Results: Endospore Isolation from Lysed Vegetative Cells
  • 08:26Conclusion

Summary

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A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

Tags

EndosporesVegetative CellsMicrobial CommunitySedimentResistanceChemicalsHeatNon-destructiveCultivationQuantificationMetabolic TestingEcologyEndospore Forming BacteriaExobiologyFood IndustrySodium HexametaphosphateSHMPHomogenizerCentrifugationFiltrationNitrocellulose Membrane

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