Plant cell wall structure and chemistry traits are evaluated to identify ideal feedstocks for biofuels and bio-materials. Standard methods have limitations when applied to large data sets. These high-throughput pretreatment, enzyme saccharification, and pyrolysis-molecular beam mass spectrometry methods compare large numbers of biomass samples with decreased experimental time and cost.
Decker, S. R., Sykes, R. W., Turner, G. B., Lupoi, J. S., Doepkke, C., Tucker, M. P., Schuster, L. A., Mazza, K., Himmel, M. E., Davis, M. F., Gjersing, E. High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release. J. Vis. Exp. (103), e53163, doi:10.3791/53163 (2015).