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High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
JoVE Journal
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High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

9,831 Views

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11:31 min

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September 15, 2015

DOI:

10.3791/53163-v

DOI:
10.3791/53163-v

•

11:31 min
September 15, 2015

•

2 Views
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1BioEnergy Science Center,National Renewable Energy Laboratory

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Plant cell wall structure and chemistry traits are evaluated to identify ideal feedstocks for biofuels and bio-materials. Standard methods have limitations when applied to large data sets. These high-throughput pretreatment, enzyme saccharification, and pyrolysis-molecular beam mass spectrometry methods compare large numbers of biomass samples with decreased experimental time and cost.

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High-throughput ScreeningLignocellulosic BiomassTotal LigninLignin MonomersEnzymatic Sugar ReleaseCellulosePyrolysis-molecular Beam Mass SpectrometryBiyoyakıtlarBiochemicalsRenewable Energy

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Decker, S. R., Sykes, R. W., Turner, G. B., Lupoi, J. S., Doepkke, C., Tucker, M. P., Schuster, L. A., Mazza, K., Himmel, M. E., Davis, M. F., Gjersing, E. High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release. J. Vis. Exp. (103), e53163, doi:10.3791/53163 (2015).

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