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总木质素，木质素单体，和酶促糖推出：顽拗变奏曲在木质纤维素生物质的高通量筛选
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High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
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总木质素，木质素单体，和酶促糖推出：顽拗变奏曲在木质纤维素生物质的高通量筛选

9,828 Views

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11:31 min

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September 15, 2015

DOI:

10.3791/53163-v

DOI:
10.3791/53163-v

•

11:31 min
September 15, 2015

•

2 Views
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1BioEnergy Science Center,National Renewable Energy Laboratory

Summary

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Plant cell wall structure and chemistry traits are evaluated to identify ideal feedstocks for biofuels and bio-materials. Standard methods have limitations when applied to large data sets. These high-throughput pretreatment, enzyme saccharification, and pyrolysis-molecular beam mass spectrometry methods compare large numbers of biomass samples with decreased experimental time and cost.

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High-throughput ScreeningLignocellulosic BiomassTotal LigninLignin MonomersEnzymatic Sugar ReleaseCellulosePyrolysis-molecular Beam Mass SpectrometryBiofuelsBiochemicalsRenewable Energy

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Cite this Article

Decker, S. R., Sykes, R. W., Turner, G. B., Lupoi, J. S., Doepkke, C., Tucker, M. P., Schuster, L. A., Mazza, K., Himmel, M. E., Davis, M. F., Gjersing, E. High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release. J. Vis. Exp. (103), e53163, doi:10.3791/53163 (2015).

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