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qPCRTag分析 - 高スループット、Sc2.0ジェノタイピングのためのリアルタイムPCRアッセイ
JoVE Journal
Biologia
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JoVE Journal Biologia
qPCRTag Analysis – A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
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qPCRTag分析 - 高スループット、Sc2.0ジェノタイピングのためのリアルタイムPCRアッセイ

16,466 Views

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07:00 min

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May 25, 2015

DOI:

10.3791/52941-v

DOI:
10.3791/52941-v

•

07:00 min
May 25, 2015

•

43 Views
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1Department of Biochemistry and Molecular Pharmacology,Institute for Systems Genetics, 2Roche Life Science, USA

Summary

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Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.

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QPCRTag AnalysisHigh ThroughputReal-time PCRSc2.0 GenotypingSynthetic Yeast GenomePCRTagOpen Reading FrameORFSynthetic ChromosomeWild Type ChromosomeGenome EngineeringMultiplex PCR

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Cite this Article

Mitchell, L. A., Phillips, N. A., Lafont, A., Martin, J. A., Cutting, R., Boeke, J. D. qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping. J. Vis. Exp. (99), e52941, doi:10.3791/52941 (2015).

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