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长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
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长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用

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15:57 min

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July 11, 2015

DOI:

10.3791/52833-v

DOI:
10.3791/52833-v

•

15:57 min
July 11, 2015

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1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine,Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service,United States Department of Agriculture

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Interferon-γELISPOT AssayEffector T CellsMemory T CellsCentral Memory T CellsEffector Memory T CellsCattleTuberculosisVaccinationAntigen StimulationLong-term Culture

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Maggioli, M. F., Palmer, M. V., Vordermeier, H. M., Whelan, A. O., Fosse, J. M., Nonnecke, B. J., Waters, W. R. Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle. J. Vis. Exp. (101), e52833, doi:10.3791/52833 (2015).

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