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Studerer Bukspyttkjertelkreft Stem Cell Kjennetegn for å utvikle nye behandlingsstrategier
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
DOI:

07:29 min

June 20, 2015

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Capitoli

  • 00:05Titolo
  • 01:28Isolation of Cancer Stem Cells from Pancreatic Ductal Adenocarcinoma
  • 02:58Sphere Formation Assay and Metformin Treatment
  • 04:21Metabolism Analysis: ROS Production and Oxygen Consumption
  • 06:09Results: The Effects of Metformin on Pancreatic CSC Function
  • 06:59Conclusion

Summary

Traduzione automatica

Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.

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