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कोशिकी डीएनए रिलीज के उच्च throughput मापन और मानव न्यूट्रोफिल में मात्रात्मक नेट गठन<em> इन विट्रो</em
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

कोशिकी डीएनए रिलीज के उच्च throughput मापन और मानव न्यूट्रोफिल में मात्रात्मक नेट गठन<em> इन विट्रो</em

DOI:

11:03 min

June 18, 2016

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Capitoli

  • 00:05Titolo
  • 00:49Isolation of Neutrophils from Peripheral Human Blood
  • 02:17Measurement of Kinetics of Extracellular DNA Release Using Microplate Fluorimeter
  • 04:22Quantitation of NET Formation by MPO-DNA and HNE-DNA ELISA Assays
  • 08:22Results: MPO-DNA and HNE-DNA ELISA Assay Results
  • 10:25Conclusion

Summary

Traduzione automatica

High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.

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