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Inmunología y contagio
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High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

DOI:
10.3791/52779-v

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11:03 min

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June 18, 2016

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, , , ,
1Department of Infectious Diseases,University of Georgia

Capítulos

  • 00:05Título
  • 00:49Isolation of Neutrophils from Peripheral Human Blood
  • 02:17Measurement of Kinetics of Extracellular DNA Release Using Microplate Fluorimeter
  • 04:22Quantitation of NET Formation by MPO-DNA and HNE-DNA ELISA Assays
  • 08:22Results: MPO-DNA and HNE-DNA ELISA Assay Results
  • 10:25Conclusion

Summary

Traducción Automática

Traducción Automática

High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.

Tags

Extracellular DNANET FormationNeutrophilsInflammationHigh ThroughputSYTOX OrangePMAPercoll GradientCell Isolation

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