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新一代测序为可操作的突变在固体和液体瘤检测
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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新一代测序为可操作的突变在固体和液体瘤检测

23,787 Views

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11:15 min

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September 20, 2016

DOI:

10.3791/52758-v

DOI:
10.3791/52758-v

•

11:15 min
September 20, 2016

•

44 Views
, , , , , , , , , , , ,
1Department of Pathology and Laboratory Medicine,Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine,Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center

Summary

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This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.

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Cite this Article

Fox, A. J., Hiemenz, M. C., Lieberman, D. B., Sukhadia, S., Li, B., Grubb, J., Candrea, P., Ganapathy, K., Zhao, J., Roth, D., Alley, E., Loren, A., Morrissette, J. J. D. Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors. J. Vis. Exp. (115), e52758, doi:10.3791/52758 (2016).

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