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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
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JoVE Journal Biologia dello sviluppo
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

12,014 Views

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08:26 min

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May 14, 2015

DOI:

10.3791/52732-v

DOI:
10.3791/52732-v

•

08:26 min
May 14, 2015

•

12 Views
,
1Division of Genetics and Development,Toronto Western Research Institute and Krembil Neuroscience Center, 2Department of Surgery, Division of Neurosurgery,Toronto Western Hospital and University of Toronto

Summary

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The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.

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Mothe, A., Tator, C. H. Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. J. Vis. Exp. (99), e52732, doi:10.3791/52732 (2015).

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