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HPLC-Messung der DNA-Oxidation Biomarker, 8-oxo-7,8-dihydro-2'-Desoxyguanosin, in kultivierten Zellen und tierischen Geweben
JoVE Journal
Chimica
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JoVE Journal Chimica
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
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HPLC-Messung der DNA-Oxidation Biomarker, 8-oxo-7,8-dihydro-2'-Desoxyguanosin, in kultivierten Zellen und tierischen Geweben

25,985 Views

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14:12 min

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August 01, 2015

DOI:

10.3791/52697-v

DOI:
10.3791/52697-v

•

14:12 min
August 01, 2015

•

7 Views
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1Environmental Health Science and Research Bureau,Health Canada

Summary

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Das Ziel des Protokolls ist die Detektion der DNA-Oxidation Marker, 8-oxo-7,8-dihydro-2'-Desoxyguanosin (8-oxo-dGuo) durch HPLC-ED, in DNA aus kultivierten Zellen oder tierische Gewebe.

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Cite this Article

Chepelev, N. L., Kennedy, D. A., Gagné, R., White, T., Long, A. S., Yauk, C. L., White, P. A. HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues. J. Vis. Exp. (102), e52697, doi:10.3791/52697 (2015).

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