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该DNA氧化标志物的高效液相色谱法测量，8-氧代-7,8-二氢-2'-脱氧鸟苷，在培养细胞和动物组织
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Chimica
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HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
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该DNA氧化标志物的高效液相色谱法测量，8-氧代-7,8-二氢-2'-脱氧鸟苷，在培养细胞和动物组织

25,985 Views

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14:12 min

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August 01, 2015

DOI:

10.3791/52697-v

DOI:
10.3791/52697-v

•

14:12 min
August 01, 2015

•

7 Views
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1Environmental Health Science and Research Bureau,Health Canada

Summary

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该协议的目的是将DNA氧化标志物的检测，8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧dGuo）通过HPLC-ED，从培养的细胞或动物组织的DNA。

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Cite this Article

Chepelev, N. L., Kennedy, D. A., Gagné, R., White, T., Long, A. S., Yauk, C. L., White, P. A. HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues. J. Vis. Exp. (102), e52697, doi:10.3791/52697 (2015).

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