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荧光标记基因的基因枪转化成机会性真菌病原<em>新型隐球菌</em

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

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荧光标记基因的基因枪转化成机会性真菌病原<em>新型隐球菌</em

DOI:

10.3791/52666-v

•

07:32 min

•

March 19, 2015

•

Tonya Taylor, Indrani Bose, Taylor Luckie, Kerry Smith

¹Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC),Clemson University, ²Department of Biology,Western Carolina University

Capitoli

00:05Titolo
01:07DNA Preparation
02:41Gene Gun Operation
04:59Plating Transformed Cells
05:52Results: Successful Homologous Recombination
07:04Conclusion

Summary

Traduzione automatica

生物射弹转化是用于产生的DNA的稳定整合入条件致病菌新生隐球菌通过同源重组的基因组中的方法。我们将展示一个结构，它具有融合到荧光标记mCherry到新生隐球菌基因编码醋酸激酶的基因枪转化。

Tags

Biolistic Transformation Fluorescent Tagged Gene Cryptococcus Neoformans Opportunistic Fungal Pathogen Acetate Production Xylulose-5-phosphate/fructose-6-phosphate Phosphoketolase Acetate Kinase MCherry Homologous Recombination Fungal Meningitis

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