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엔테로 바이러스 및 문화와 RT-qPCR에 의해 물에서 노로 바이러스의 발생의 EPA 방법 1615 측정. 파트 III. RT-qPCR에 의한 바이러스 검출
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
DOI:

12:32 min

January 16, 2016

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Capitoli

  • 00:05Titolo
  • 02:03Tertiary Concentration and Nucleic Acid Isolation
  • 03:21Reverse Transcription
  • 05:57Real Time Quantitative PCR
  • 09:23Results: RT-qPCR Determination of Enterovirus and Norovirus in Water
  • 11:38Conclusion

Summary

Traduzione automatica

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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