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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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Biologia
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JoVE Journal Biologia
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

DOI:
10.3791/52568-v

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09:44 min

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March 03, 2015

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, ,
1Laboratory of Translational Genomics, Centre for Integrative Biology,University of Trento, 2High Throughput Screening Core Facility, Centre for Integrative Biology,University of Trento

Capitoli

  • 00:05Titolo
  • 01:30Day 1: Prepare and Seed Cells
  • 02:44Day 2: Treat the Cells with Library Compounds
  • 04:19Day 3: Luciferase Assay
  • 05:27Counter-screening
  • 07:09Results: Modulators of the 3′ UTR of MYCN
  • 09:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

Tags

Keywords: High-throughput ScreeningChemical ModulatorsPost-transcriptional RegulationGene Expression3′ Untranslated RegionLuciferase ReporterCell-based AssayPrimary ScreeningCounter-screeningPost-transcriptional Mechanisms

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