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JoVE Journal Neuroscience
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
 

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

Article DOI: 10.3791/52551-v • 09:24 min • April 22nd, 2015
April 22nd, 2015 •
*1, *1, 1, 1
1Hans Berger Department of Neurology, Jena University Hospital
* These authors contributed equally

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This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

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Immunohistochemistry Multiple Labeling Antibodies Same Host Species Adult Hippocampal Neurogenesis Molecular Markers Morphological Criteria Immunofluorescent Techniques Proliferation Markers Thymidine Analogs Transcardial Perfusion Tissue Processing Heat-induced Epitope Retrieval ABC Immunohistochemistry Multiple Indirect Immunofluorescence Confocal Microscopy Cell Quantification Sequential Multiple Immunofluorescence Protocol
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