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Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes
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Neuroscienze
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JoVE Journal Neuroscienze
Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

DOI:
10.3791/50987-v

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10:55 min

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May 03, 2014

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, , ,
1Laboratory of Neurodevelopement and Repair,University of Science and Technology of China

Capitoli

  • 00:00Titolo
  • 00:55Introduction
  • 01:17Construct the apparatus
  • 01:47Prepare reagents and tools
  • 03:20Begin the surgery
  • 07:26Wholemounting retina and imaging
  • 10:30Representative results

Summary

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We introduce an efficient method to retrograde label retinal ganglion cells (RGCs) in adult zebrafish.

Tags

Retrograde LabelingRetinal Ganglion CellsRGCsFluorescent DyesAdult ZebrafishOptic Nerve InjuryRGCs SurvivalRGCs RegenerationAcid-etchingLight Curing BondOptic Nerve Crush

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