/
/
Ex Utero electroporación y organotípicos rebanada Cultura de ratón Hipocampo Tissue
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:17DNA Injection and Electroporation of E15.5 and E18.5 Embryos
- 03:13Dissection of the Brain
- 04:21Vibratiome Sectioning and Slice Culture
- 06:07Fixation of the Sections Followed by Immunofluorescence Staining
- 07:48Results: Ex Utero Electroporation at E18.5 Following by Organotypic Slice Culture
- 08:49Conclusion
Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.
