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Quantitative Mass Spectrometric Profiling de proteomas de células do cancro da derivadas do líquido e tumores sólidos
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February 27, 2015
DOI:
1Institute of Pathology,University Medical Center, Göttingen, 2Department of Hematology/Oncology,Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group,Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry,University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center
Summary
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In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.
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Bohnenberger, H., Ströbel, P., Mohr, S., Corso, J., Berg, T., Urlaub, H., Lenz, C., Serve, H., Oellerich, T. Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors. J. Vis. Exp. (96), e52435, doi:10.3791/52435 (2015).
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