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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
 

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Article DOI: 10.3791/52435-v • 08:08 min • February 27th, 2015
February 27th, 2015 •
1, 1, 2, 3, 2, 3,4, 3,4, 2,5,6, 2,5,6
1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center

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In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.

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Quantitative Mass Spectrometric Profiling Cancer-cell Proteomes Liquid Tumors Solid Tumors Oncogenic Pathomechanisms Drug Targets Diagnostic Biomarkers Proteomic Characterization Patient-derived Tumors Cellular Subpopulations Super-SILAC-based Mass Spectrometry Bioinformatic Data Analysis Cellular Enrichment Strategies Flow Cytometry FACS-sorting Laser-capture Microdissection Protein Extraction SILAC-labeled Spike-in Standard Gel Electrophoresis Filter Aided Sample Preparation (FASP) Tryptic Digestion Hybrid Quadrupole-orbitrap Mass Spectrometer
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