In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.
Bryan, A. M., Farnoud, A. M., Mor, V., Del Poeta, M. Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans. J. Vis. Exp. (94), e52432, doi:10.3791/52432 (2014).