Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Louise E. Bird; Heather Rada; Anil Verma; Raphael Gasper; James Birch; Matthew Jennions; Jan L&#1255;we; Isabel Moraes; Raymond J. Owens

doi:10.3791/52357

Rivista

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Biologia

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膜蛋白的绿色荧光蛋白为基础的表达筛选大肠杆菌</em

JoVE Journal
Biologia

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JoVE Journal Biologia

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Please note that all translations are automatically generated. Click here for the English version.

膜蛋白的绿色荧光蛋白为基础的表达筛选大肠杆菌</em

DOI:

10.3791/52357-v

•

08:46 min

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January 06, 2015

•

Louise E. Bird, Heather Rada, Anil Verma, Raphael Gasper³, James Birch, Matthew Jennions, Jan Lӧwe, Isabel Moraes, Raymond J. Owens

¹Oxford Protein Production Facility,Research Complex at Harwell, ²Protein Crystallography Group,Ruhr University, ³MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, ⁴Membrane Protein Laboratory,Diamond Light Source

Capitoli

00:05Titolo
01:58Analysis of Isopropyl Beta-D-1-thiogalactopyranoside (IPTG)-induced Expression by In-gel Flourescence
03:37Scale-up of Cell Cultures and Membrane Preparation
05:30Solubilization and Analysis of the Membrane Fraction
06:48Results: Representative In-gel and FSEC Analyses
08:11Conclusion

Summary

Traduzione automatica

简化的方法来筛选重组膜蛋白的基础上融合绿色荧光蛋白大肠杆菌表达呈现。

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