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Capitoli
- 00:05Titolo
- 01:28Culture of Organotypic Bronchial Tissue
- 02:04In vitro Cigarette Smoke (CS) Exposure
- 03:22Measurement of Ciliary Beating & Transepithelial Electrical Resistance (TEER)
- 04:20Cytochrome P450 (CYP) 1A1/1B1 Activity
- 05:02RNA Extraction
- 06:35Microarray Workflow & Washing and Staining
- 08:07Results: Applicability of Human Organotypic Tissue Models
- 09:03Conclusion
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
