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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
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December 23, 2014
DOI:
1Department of Biology,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, 2Department of Chemistry and Biochemistry,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University, 3Department of Exercise Science,Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University
Summary
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Periodic acid Schiff staining is a technique that visualizes the polysaccharide content of tissues. This article demonstrates periodic acid Schiff staining protocol adapted for use on peripheral blood mononuclear cells purified from human venous blood. Such samples are enriched for lymphocytes and other white blood cells of the immune system.
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Tabatabaei Shafiei, M., Carvajal Gonczi, C. M., Rahman, M. S., East, A., François, J., Darlington, P. J. Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining. J. Vis. Exp. (94), e52199, doi:10.3791/52199 (2014).
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