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एक मॉडल के रूप घ्राण प्रणाली axonal विकास पैटर्न और आकृति विज्ञान का अध्ययन करने के लिए<em> Vivo</em
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
DOI:

08:29 min

October 30, 2014

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Capitoli

  • 00:05Titolo
  • 01:20Preparation of Instruments and Pipette Fabrication
  • 02:43Preparation of Electroporation Solution
  • 03:32Electroporation of Fluorophore-coupled Dextrans
  • 05:27Mounting Animals for In Vivo Visualization of Cells and Axonal Processes
  • 06:44Results: Visualizing Olfactory Axon Projections in the Excised Olfactory Bulb
  • 08:05Conclusion

Summary

Traduzione automatica

We describe a protocol for in vivo labeling of olfactory sensory neurons by electroporation and subsequent confocal laser-scanning or multiphoton microscopy to visualize neuronal morphology and its development over time.

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