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Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

60,169 Views

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13:26 min

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September 13, 2014

DOI:

10.3791/51809-v

DOI:

10.3791/51809-v

•

13:26 min

September 13, 2014

•

12 Views

Mirella Vivoli, Halina R. Novak, Jennifer A. Littlechild, Nicholas J. Harmer

¹Department of Biosciences,University of Exeter

Tags

Protein-ligand Interactions Differential Scanning Fluorimetry Dissociation Constant Binding Affinity PCR Machine Quantitative PCR Hit Discovery Binding Models Cooperative Binding Independent Binding Sites

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