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Capitoli
- 00:05Titolo
- 01:52Labeling and Injection of HSPCs
- 03:21Preparing the Mouse for Imaging
- 05:20In Vivo Imaging: High-resolution Stacks and Time-lapse Acquisition
- 11:25Results: In Vivo 4D Imaging of HSPCs in Mouse Calvarium
- 12:16Conclusion
The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.
