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Immunology and Infection

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JoVE Journal Immunology and Infection
A Protocol for Analyzing Hepatitis C Virus Replication
 

A Protocol for Analyzing Hepatitis C Virus Replication

Article DOI: 10.3791/51362-v • 13:04 min • June 26th, 2014
June 26th, 2014 •
*1, *1, 1,2
1Liver Program at Regenerative Medicine Institute, Department of Biomedical Sciences, Department of Surgery, Cedars-Sinai Medical Center, 2Department of Surgery, David Geffen School of Medicine at UCLA
* These authors contributed equally

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Hepatitis C Virus (HCV) is a major human pathogen that causes liver disorders, including cirrhosis and cancer. An HCV infectious cell culture system is essential for understanding the molecular mechanism of HCV replication and developing new therapeutic approaches. Here we describe a protocol to investigate various stages of the HCV replication cycle.

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Hepatitis C Virus HCV Replication Protocol Analyzing Viral Entry Genome Replication Packaging Egress Cell Culture System FNX-HCV FNX-Rluc Virus Renilla Luciferase Reporter Gene Human Hepatoma Cell Line In Vitro Transcribed HCV Genomic RNAs Quantitative RT-PCR HCV Double-stranded RNA Western Blot Immunofluorescence Assays HCV NS3 Protein HCV NS5A Protein Infectious Particles Viral Titer Luciferase Assays
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