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Immunologia e infezioni

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生发中心的高度已解决活体条纹照明显微镜

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

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生发中心的高度已解决活体条纹照明显微镜

DOI:

10.3791/51135-v

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10:07 min

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April 09, 2014

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Zoltan Cseresnyes*^1,2, Laura Oehme*³, Volker Andresen, Anje Sporbert, Anja E. Hauser*^3,5, Raluca Niesner*¹

¹Biophysical Analytics, German Rheumatism Research Center,Leibniz Institute, ²Microscopy Core Facility,Max-Delbrück Center for Molecular Medicine, ³Immunodynamics, German Rheumatism Research Center,Leibniz Institute, ⁴LaVision Biotec GmbH, ⁵Immunodynamics and Intravital Imaging,Charité – University of Medicine

Capitoli

00:05Titolo
01:26Multi-beam Setup for Striped-illumination TPLSM
06:07Results: Highly-resolved Intravital Striped-illumination Images
09:05Conclusion

Summary

Traduzione automatica

高分辨率的活体成像具有增强对比度高达120微米的深度在成年小鼠的淋巴结被空间调制多焦点双光子显微镜的激励模式来实现。在100微米的深度，我们测量了487 nm的分辨率（横向）和551纳米（轴向），从而绕过散射和衍射极限。

Tags

Intravital Microscopy Striped-illumination Multi-photon Microscopy Germinal Centers Immune Cell Dynamics Lymphoid Organs High-resolution Signal-to-noise Ratio Imaging Depth

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