Rivista
/
Immunologia e infezioni
/
El uso de proteínas fluorescentes para visualizar y cuantificar<em> Chlamydia</em> Vacuola dinámica de crecimiento en las células vivas
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Please note that all translations are automatically generated. Click here for the English version.

El uso de proteínas fluorescentes para visualizar y cuantificar<em> Chlamydia</em> Vacuola dinámica de crecimiento en las células vivas

7,414 Views

•

07:42 min

•

October 13, 2015

DOI:

10.3791/51131-v

DOI:
10.3791/51131-v

•

07:42 min
October 13, 2015

•

2 Views
, ,
1Division of Allergy and Infectious Diseases, Department of Medicine,University of Washington, 2Program in Infectious Diseases, School of Public Health,University of California at Berkeley

Summary

Automatically generated

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

Explore More Videos

ChlamydiaInclusionVacuoleFluorescent ProteinsGFPMCherryVisualizationQuantitationHost CellIntracellularImagingReverse-imagingAntibody-based Enumeration

Read Article

Cite this Article

Zuck, M., Feng, C., Hybiske, K. Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells. J. Vis. Exp. (104), e51131, doi:10.3791/51131 (2015).

Download .ris file

Copy

Share Video

.

Copy