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Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
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JoVE Journal Chimica
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

DOI:
10.3791/50969-v

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10:02 min

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February 18, 2014

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1Department of Chemistry and Biochemistry,Florida International University

Capitoli

  • 00:05Titolo
  • 01:42Experimental Setup
  • 05:18PBD Measurements
  • 06:28Data Analysis
  • 08:17Results: Representative PBD Traces
  • 09:28Conclusion

Summary

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Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

Tags

Photothermal Beam DeflectionPhoto-acoustic CalorimetryThermal GratingConformational ChangesProteinsMicrosecond To Millisecond Time-scalesVolume ChangesEnthalpy ChangesCalcium BindingCalcium TransducersNeuronal Calcium SensorsDM-nitrophenPhoto-triggeringFast Kinetics

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