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Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
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JoVE Journal Chimica
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

8,849 Views

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10:02 min

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February 18, 2014

DOI:

10.3791/50969-v

DOI:
10.3791/50969-v

•

10:02 min
February 18, 2014

•

2 Views
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1Department of Chemistry and Biochemistry,Florida International University

Summary

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Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

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Photothermal Beam DeflectionPhoto-acoustic CalorimetryThermal GratingConformational ChangesProteinsMicrosecond To Millisecond Time-scalesVolume ChangesEnthalpy ChangesCalcium BindingCalcium TransducersNeuronal Calcium SensorsDM-nitrophenPhoto-triggeringFast Kinetics

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Cite this Article

Gonzalez, W. G., Miksovska, J. Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection. J. Vis. Exp. (84), e50969, doi:10.3791/50969 (2014).

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