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A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Capitoli
- 00:05Titolo
- 02:14Collagen Precoating of Microporous Inserts
- 03:44Culture of Endothelial Cells on the Upper Inserts
- 04:573D Flow Chamber Assembly and Priming
- 07:59Circulating Cell Assay and Sample Collection
- 09:19Risultati
- 10:26Conclusion
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
