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Bioengineering

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JoVE Journal Bioengineering
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
 

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

Article DOI: 10.3791/50959-v • 10:56 min • July 15th, 2013
July 15th, 2013 •
1, 1,2
1Torrey Pines Institute for Molecular Studies, 2Cascade LifeSciences Inc.

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The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.

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Three-dimensional Flow Chamber Device Chemokine-directed Extravasation Cells Circulating Physiological Flow Conditions In Vitro Technology Shear Stress Cell Extravasation Cascade Upper Compartment Lower Compartment Chemoattractants Porous Inserts Monolayer Of Endothelial Cells Microenvironmental Cells Gas Exchange Unit CO2 Tension Peristaltic Pump Circulating Cells Migrated Cells Cell Rolling Adhesion To EC Transmigration Chemokine Gradients Resistance To Shear
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