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Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
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Capitoli
- 00:05Titolo
- 01:45Spinal Cord Dissection
- 05:03FP Culture and FP Conditioned Medium Harvesting
- 06:35Commissural Neuron Dissection and Treatments
- 08:15Results: Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
- 08:58Conclusion
The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.
