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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
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JoVE Journal Neuroscienze
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

10,731 Views

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09:42 min

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December 08, 2013

DOI:

10.3791/50794-v

DOI:
10.3791/50794-v

•

09:42 min
December 08, 2013

•

4 Views
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1Center for Research on Occupational and Environmental Toxicology,Oregon Health & Science University, 2Department of Behavioral Neuroscience,Oregon Health & Science University

Summary

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Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

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Cite this Article

Irwin, R. P., Allen, C. N. Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons. J. Vis. Exp. (82), e50794, doi:10.3791/50794 (2013).

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