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Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

10,910 Views

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15:31 min

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August 16, 2013

DOI:

10.3791/50723-v

DOI:
10.3791/50723-v

•

15:31 min
August 16, 2013

•

1 Views
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1Department of Immunology and Infectious Diseases,Montana State University, 2Department of Molecular Biology,Princeton University

Summary

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Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

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Anterograde TransportLive Cell ImagingFluorescent Fusion ProteinsViral Particle AssembliesCell-cell SpreadParticle Transport VelocitiesProtein LocalizationViral Genetic TechniquesAlphaherpes Virus

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Cite this Article

Taylor, M. P., Kratchmarov, R., Enquist, L. W. Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread. J. Vis. Exp. (78), e50723, doi:10.3791/50723 (2013).

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